1. 研究方向
深圳市肿瘤代谢组学重点实验室的主要研究方向为肿瘤代谢组学研究，重点研究肿瘤疾病的发生机理、早期诊断和预测预警以及抗肿瘤药物研发。主要研究内容： 1、研究基因调控网络系统和代谢调控途径，识别和鉴定在癌症发生发展初期起关键作用的生物标记物，将研究结果转化为临床应用，形成肿瘤预警检测和治疗系统； 2、研究与上述生物标记物的代谢通路中有相互关系的关键调控物质为靶点，设计合成抗肿瘤小分子抑制剂。
2. 建设目标
围绕肿瘤的代谢组学研究，开展肿瘤细胞信号传导通路研究，探寻细胞癌变过程中基因、蛋白质间相互调控关系，构建肿瘤发生发展的多靶点调控网络；研究肿瘤细胞的代谢产物变化，探讨与肿瘤发生和生长相关的生物标记物，建立肿瘤疾病的预警和治疗系统研究平台。形成以我国为主导的新的食品药品安全性评价标准，率先形成从肿瘤的预防检测到治疗的新兴产业链。
3. 科研条件
深圳肿瘤代谢组学重点实验室自 2010 年开始建设，现已搭建完善了组学研究平台（小分子代谢组学、大分子蛋白质组学和基因芯片等）、化合物结构鉴定技术平台、生物学功能验证平台、生物信息计算平台和临床医学研究平台等，形成了国际先进的肿瘤代谢组学研究平台。目前，实验场地 600 平方米，固定资产 1900 余万元，研究团队 31 人，其中专职科研人员 28 人，博士学位以上人员占 70%以上，管理人员 3 人，客座教授 4 人。
4. 研究成果
代表性论文：
1.Gao, X.; Bi, X.; Wei, J. T.; Peng, Z. M.; Liu, H. X.; Jiang, Y. Y.; Wei, W.; Cai, Z. W., N-phosphorylation labeling for analysis of twenty natural amino acids and small peptides by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Analyst 2013,138 (9), 2632-2639.
2.Gao, X.; Wu, H. Z.; Lee, K. C.; Liu, H. X.; Zhao, Y. F.; Cai, Z. W.; Jiang, Y. Y., Stable Isotope N-Phosphorylation Labeling for Peptide de Novo Sequencing and Protein Quantification Based on Organic Phosphorus Chemistry. Anal Chem 2012,84 (23), 10236-10244.
3.Li, S. F.; Jin, Y. B.; Tang, Z.; Lin, S. H.; Liu, H. X.; Jiang, Y. Y.; Cai, Z. W., A novel method of liquid chromatography-tandem mass spectrometry combined with chemical derivatization for the determination of ribonucleosides in urine. Anal Chim Acta 2015,864, 30-38.
4.Wang, Y.; Gao, D.; Chen, Z.; Li, S.; Gao, C.; Cao, D.; Liu, F.; Liu, H.; Jiang, Y., Acridone derivative 8a induces oxidative stress-mediated apoptosis in CCRF-CEM leukemia cells: application of metabolomics in mechanistic studies of antitumor agents. Plos One 2013,8 (5), e63572.
5.Bi, X.; Jin, Y.; Li, S.; Gao, D.; Jiang, Y.; Liu, H., Rapid and sensitive determination of fatty acids in edible oil by liquid chromatography-electrospray ionization tandem mass spectrometry. Science China Chem 2013,57 (3), 447-452.
6.Bi, X.; Jin, Y.; Gao, X.; Liu, F.; Gao, D.; Jiang, Y.; Liu, H., Investigation of Pokemon-Regulated Proteins in Hepatocellular Carcinoma Using Mass Spectrometry-Based Multiplex Quantitative Proteomics. Eur J Mass Spectrom 2013,19 (2), 111-121.
7.Gao, D.; Jin, F.; Liu, H.; Wang, Y.; Jiang, Y., Metabonomic study on the antitumor effect of flavonoid derivative 3d in HepG2 cells and its action mechanism. Talanta 2014,118, 382-8.
8.Wei, J.; Jin, F.; Wu, Q.; Jiang, Y.; Gao, D.; Liu, H., Molecular interaction study of flavonoid derivative 3d with human serum albumin using multispectroscopic and molecular modeling approach. Talanta 2014,126, 116-21.
9.Chen, X.; Gao, D.; Liu, F.; Gao, X.; Wang, S.; Zhao, Y.; Liu, H.; Jiang, Y., A novel quantification method for analysis of twenty natural amino acids in human serum based on N-phosphorylation labeling using reversed-phase liquid chromatography-tandem mass spectrometry. Anal Chim Acta 2014,836, 61-71.
10.Xie, W.; Gao, D.; Jin, F.; Jiang, Y.; Liu, H., Study of Phospholipids in Single Cells Using an Integrated Microfluidic Device Combined with Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry. Anal Chem 2015,87 (14), 7052-9.
11.Gao, D.; Chen, X.; Yang, X.; Wu, Q.; Jin, F.; Wen, H.; Jiang, Y.; Liu, H., Stable isotope labeling strategy for curcumin metabolite study in human liver microsomes by liquid chromatography-tandem mass spectrometry. J Am Soc Mass Spectrom 2015,26 (4), 686-94.
12.Chen, Y.; Gao, D.; Liu, H.; Lin, S.; Jiang, Y., Drug cytotoxicity and signaling pathway analysis with three-dimensional tumor spheroids in a microwell-based microfluidic chip for drug screening. Anal Chim Acta 2015,898, 85-92.
13.Wang, Y.; Gao, D.; Chu, B.; Gao, C.; Cao, D.; Liu, H.; Jiang, Y., Exposure of CCRF-CEM cells to acridone derivative 8a triggers tumor death via multiple mechanisms. Proteomics 2016,16 (7), 1177-90.
14.Shao, X.; Gao, D.; Wang, Y.; Jin, F.; Wu, Q.; Liu, H., Application of metabolomics to investigate the antitumor mechanism of flavopiridol in MCF-7 breast cancer cells. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 2016,1025, 40-7.
15.Gao, D.; Wang, Y.; Xie, W.; Yang, T.; Jiang, Y.; Guo, Y.; Guan, J.; Liu, H., Metabolomics study on the antitumor effect of marine natural compound flexibilide in HCT-116 colon cancer cell line. J Chromatogr B 2016,1014, 17-23.
16.Bai, H.; Wang, S.; Liu, J.; Gao, D.; Jiang, Y.; Liu, H.; Cai, Z., Localization of ginsenosides in Panax ginseng with different age by matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry imaging. J Chromatogr B 2016,1026, 263-271.
17.Song, L.; Gao, D.; Li, S.; Wang, Y.; Liu, H.; Jiang, Y., Simultaneous quantitation of hydrazine and acetylhydrazine in human plasma by high performance liquid chromatography-tandem mass spectrometry after derivatization with p-tolualdehyde. J Chromatogr B 2017,1063, 189-195.
18.Liu, W.; Jin, F.; Gao, D.; Song, L.; Ding, C.; Liu, H., Metabolomics analysis reveals aminoquinazolin derivative 9d-induced oxidative stress and cell cycle arrest in A549 cells. Rsc Adv 2017,7 (22), 13149-13158.
19.Wang, N.; Zhang, B.; Jin, F.; Gao, D.; Liu, F.; Liu, H.; Jiang, Y., Combing metabolomics with bioanalysis methods to study the antitumor mechanism of the new acridone derivative 8q on CCRF-CEM cells: 8q induced mitochondrial-mediated apoptosis and targeted the PI3K/AKT/FOXO1 pathway. J Pharm Biomed Anal 2018,160, 314-322.
20.Wang, N.; Chen, S.; Zhang, B.; Li, S.; Jin, F.; Gao, D.; Liu, H.; Jiang, Y., 8u, a pro-apoptosis/cell cycle arrest compound, suppresses invasion and metastasis through HSP90alpha downregulating and PI3K/Akt inactivation in hepatocellular carcinoma cells. Sci Rep 2018,8 (1), 309.
21.Li, S.; Gao, D.; Song, C.; Tan, C.; Jiang, Y., Isotope Labeling Strategies for Acylcarnitines Profile in Biological Samples by Liquid Chromatography-Mass Spectrometry. Anal Chem 2019,91 (3), 1701-1705.
22.Song, C.; Gao, D.; Li, S.; Liu, L.; Chen, X.; Jiang, Y., Determination and quantification of fatty acid C=C isomers by epoxidation reaction and liquid chromatography-mass spectrometry. Anal Chim Acta 2019,1086, 82-89.
23.Li, S.; Gao, D.; Jiang, Y., Function, Detection and Alteration of Acylcarnitine Metabolism in Hepatocellular Carcinoma. Metabolites 2019,9 (2).
24.Chen, X.; Gao, D.; Sun, Q.; Chen, Y.; Liu, H.; Jiang, Y., Metabolic Profiling of Amino Acids by Liquid Chromatography–Tandem Mass Spectrometry (LC–MS) to Characterize the Significance of Glutamine in Triple-Negative Breast Cancer (TNBC). Anal Lett 2019,52 (7), 1068-1082.
25.Yini Wang, C. X., Bowen Zhong, Dongdong Zhan, Mingwei Liu, Dan Gao, Yi Wang, and Jun Qin, Comparative Proteomic Analysis of Histone Modifications upon Acridone Derivative 8a-Induced CCRF-CEM Cells by Data Independent Acquisition. J. Proteome Res. 2020,19, 819−831.
专利：
1. 特异性调节POKEMON基因表达的反义寡核苷酸（ZL200710098573.2）
2. 治疗乳腺癌的药物及其专用反义寡核苷酸（200710098572.8）
3. 具有抑制PTTG表达作用的融合蛋白及其编码基因与应用（ZL200610169706.6）
4. 肽苯酰胺化合物及其制备方法 (ZL200510037250.3)
5. 二异丙氧基磷酰化二肽三甲氧基苯酰胺化合物及其制备方法(ZL200510101669.0)
6. 基于化学衍生化与HPLC-MS的不饱和脂肪酸定量方法（201910238742）
7. 联系人及联系方式
高丹：0755-26036533
